DataSheet1_Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis.docx

Introduction: Preeclampsia (PE) is a pregnancy-associated, multi-organ, life-threatening disease that appears after the 20th week of gestation. The aim of this study was to perform a systematic review and meta-analysis to determine whether women with PE have disrupted miRNA expression compared to women who do not have PE.Methods: We conducted a systematic review and meta-analysis of studies that reported miRNAs expression levels in placenta or peripheral blood of pregnant women with vs. without PE. Studies published before October 29, 2021 were identified through PubMed, EMBASE and Web of Science. Two reviewers used predefined forms and protocols to evaluate independently the eligibility of studies based on titles and abstracts and to perform full-text screening, data abstraction and quality assessment. Standardized mean difference (SMD) was used as a measure of effect size.Results: 229 publications were included in the systematic review and 53 in the meta-analysis. The expression levels in placenta were significantly higher in women with PE compared to women without PE for miRNA-16 (SMD = 1.51,95%CI = 0.55–2.46), miRNA-20b (SMD = 0.89, 95%CI = 0.33–1.45), miRNA-23a (SMD = 2.02, 95%CI = 1.25–2.78), miRNA-29b (SMD = 1.37, 95%CI = 0.36–2.37), miRNA-155 (SMD = 2.99, 95%CI = 0.83–5.14) and miRNA-210 (SMD = 1.63, 95%CI = 0.69–2.58), and significantly lower for miRNA-376c (SMD = –4.86, 95%CI = –9.51 to –0.20). An increased level of miRNK-155 expression was found in peripheral blood of women with PE (SMD = 2.06, 95%CI = 0.35–3.76), while the expression level of miRNA-16 was significantly lower in peripheral blood of PE women (SMD = –0.47, 95%CI = –0.91 to –0.03). The functional roles of the presented miRNAs include control of trophoblast proliferation, migration, invasion, apoptosis, differentiation, cellular metabolism and angiogenesis.Conclusion: miRNAs play an important role in the pathophysiology of PE. The identification of differentially expressed miRNAs in maternal blood creates an opportunity to define an easily accessible biomarker of PE.

引言：妊娠期高血压（PE）是一种与妊娠相关的多器官、危及生命的疾病，通常出现在妊娠20周之后。本研究旨在通过系统评价和荟萃分析，探究患有PE的女性与未患有PE的女性相比，其miRNA表达是否存在紊乱。方法：我们对报道妊娠期女性胎盘或外周血中miRNA表达水平的文献进行了系统评价和荟萃分析。通过PubMed、EMBASE和Web of Science数据库，检索了截至2021年10月29日之前发表的文献。两位评审员根据标题和摘要，使用预定义的表格和协议独立评估了研究的适宜性，并进行了全文筛选、数据提取和质量评估。标准化均数差（SMD）被用作效应量测度。结果：系统评价纳入了229篇文献，荟萃分析纳入了53篇。与未患有PE的女性相比，患有PE的女性在胎盘中miRNA-16（SMD = 1.51，95%CI = 0.55–2.46）、miRNA-20b（SMD = 0.89，95%CI = 0.33–1.45）、miRNA-23a（SMD = 2.02，95%CI = 1.25–2.78）、miRNA-29b（SMD = 1.37，95%CI = 0.36–2.37）、miRNA-155（SMD = 2.99，95%CI = 0.83–5.14）和miRNA-210（SMD = 1.63，95%CI = 0.69–2.58）的表达水平显著升高，而在miRNA-376c（SMD = –4.86，95%CI = –9.51至–0.20）的表达水平则显著降低。在患有PE的女性的外周血中，miRNK-155的表达水平升高（SMD = 2.06，95%CI = 0.35–3.76），而miRNA-16在外周血中的表达水平在PE女性中显著降低（SMD = –0.47，95%CI = –0.91至–0.03）。所呈现的miRNAs的功能作用包括调控滋养层增殖、迁移、侵袭、凋亡、分化、细胞代谢和血管生成。结论：miRNAs在PE的病理生理学中发挥着重要作用。在母血中识别出差异表达的miRNAs，为定义易于获取的PE生物标志物提供了机会。