o Exploring dynamics of pre-germinative metabolism in Medicago truncatula seeds during priming, dry-back, and post-priming imbibition using proteomics

o The dramatic impact of climate change poses a demanding challenge: the need to enhance seed quality by designing tailored seed priming protocols. Proteomics can provide a comprehensive picture of the molecular players behind the seed response to priming. Using label-free protein quantification with mass spectrometry, changes in the seed proteome of the model legume Medicago truncatula were investigated along the rehydration-dehydration cycle of a standard vigorization treatment (hydropriming plus dry-back) and during post-priming imbibition.