DNA sequences of PCR fragments obtained from genomic DNA of Solanum tuberosum cv. Désirée mutants in GI.04 and GI.12 genes

Earliness of tuberisation is an important agronomic trait. It was demonstrated earlier that GIGANTEA (GI), a plant-specific nuclear protein that regulates multiple processes, is indirectly involved in tuberisation in a diploid potato. Commercial potatoes, including the cultivar Désirée, are tetraploids and carry two copies of GI genes, designated GI.04 and GI.12. The aim of our study was to explore the role of the two GI genes in Désirée in relation to tuberisation. To obtain information on GI.04 and GI.12 functions in Désirée, mutations were introduced into the two genes individually and simultaneously using the CRISPR/Cas9 system. Two different segments of the genes were targeted by gRNAs. PCR was used for mutant identification. Three mutants from each mutagenesis were selected, and the mutations were localised by generating PCR fragments using primers surrounding the targeted regions and sequencing them. Sequences of the PCR fragments are available from the uploaded file.