Gene expression profile of B55a/PPP2R2A knocked-down Parkin-expressing HeLa cells

Or study identified the PP2A-B55a/PPP2R2A Ser-Thr phosphatase as a regulator of Parkin-dependent mitophagy and mitochondrial biogenesis. HeLa cells expressing Parkin and knocked-down for B55a/PPP2R2A induce a lysosome- and mitochondria-related transcriptional programmes down-stream TFEB and PGC-1a transcription factor activation. Overall design: Total RNA was extracted from B55a/PPP2R2A kncked-down cells, induced for Parkin expression with Doxycycline. Cells transiently transfected with a control oligo were used a control sample.