DENV serotypes, viremia titer, and anti-DENV antibody isotypes in DENV-infected patients.

aID, patient identification.bDengue disease was determined by examining the clinical symptoms of patients according to the WHO criteria.cDENV serotype was determined by RT-PCR with universal and serotype-specific primers [31].dViremia titers were determined in a focus-forming immunoassay in semi-adherent K562 cells [30].eIgM in patient serum was detected by Dengue Virus IgM Capture DxSelect. An index value of >1.00 was interpreted as positive (POS) and an index value of fIgG in patient serum was detected by Dengue Virus IgG DxSelect. An index value of >1.00 was interpreted as positive (POS) and an index value of gCases with an IgM/IgG index ratio of ≤1.2 were diagnosed as secondary infections [32].hPatient’s blood specimen was used for huMAb preparation as described elsewhere [24]. ND, not detectable.