Bioprocess valorization of spent sulfite liquor with Corynebacterium glutamicum on minimal medium

Fermentation data of 5 replicate batch cultivations with Corynebacterium glutamicum on low molecular weight permeate of ultra-filtered spent sulfite liquor (UF-SSL) from softwood pulping (stored at 4°C). For all experiments, C. glutamicum CR099::U pXMJ19 was used, which, in contrast to the wild-type, contains genes for improved mannose uptake and the ability to metabolize xylose integrated into the genome (denoted by ::U), as well as an empty plasmid for potential product biosynthetic genes. The valorization of UF-SSL was performed without additional prior detoxification steps such as bioling or overliming using a minimal medium based on UF-SSL.
The dataset consists of on-line data recorded during cultivation (pH, T, dO2, O2,offgas, CO2,offgas, weight of reactor and feeds, gassing, ...), off-line data of 9 samples per batch taken every 3h (BM; HPLC: glc, man, gal, ara, xyl, urea; Enzyme Assay: ace, lac, glu, NH4, PO4; ICP-MS: Na, Mg, Ca, Mn, Fe, Ni, Cu, Zn) and meta data (feed composition, quantity, density...). UF-SSL (42 g/L glucose, 135 g/L mannose, 57 g/L xylose, 29 g/L galactose, 13 g/L arabinose, 6.4 g/L acetate) was diluted with water to 25% (~10 g/L initial glucose concentration), sterile filtered (0.2µm) and supplemented (0.2 mg/L biotin, 12 mg/L chloramphenicol, 5 ml/L polyproylenglycol 2000). Trace elements were not added since sufficient amounts (relative to the defined medium) were present in the UF-SSL. Due to strong precipitation when PO4 was added to UF-SSL (containing 14 g/L Ca), a separate nitrogen and phosphate (NP) feed was used instead of mixing with the medium. The NP feed (66.4 g/L urea and 71.8 g/L KH2PO4, sterile filtered) was added at a constant feed rate ( V = 4.5 mL/h until 100 ml were fed) to the 1 L UF-SSL minimal medium batch volume in order to achive a CN ratio of ~1:10 and CP ratio of ~1:4 at full addition.