Results for S. homoeocarpa contamination of creeping bentgrass commercial seed lots by culture-based and molecular detection methods.

1Two representative lots of each cultivar were selected by for detection of S. homoeocarpa contamination by each method; additional lots of ‘Shark’ and ‘Penncross’ seed were tested by the nested PCR detection method only.2Three 1,000 seed replicates for all lots were performed by plating 500 seeds from each lot onto two different media semi-selective for S. homoecarpa (1,000 seeds total) and repeating three times (3,000 seeds/lot total).3Nested PCR was repeated twice for each seed lot; numbers shown in parentheses are the number of subsamples testing positive for S. homoeocarpa contamination in each run.4Q-PCR detection was ceased after it was determined that PCR inhibitors in seed DNA samples rendered the assay ineffective.Results for S. homoeocarpa contamination of creeping bentgrass commercial seed lots by culture-based and molecular detection methods.