Expression profile of p16High primary peritonal macrophages. Expression profile of p16High primary peritonal macrophages

In our laboratory, we developed a new knock-in murine model to trace p16 cells ex vivo using EGFP as a reporter gene under the control of the endogenous promoter (exon 3) of the p16 locus from the CDKN2A gene (p16-Cre/Rosa26-mtmg). We showed previously that cells positive for p16-EGFP accumulated during natural aging. We previously reported (Grosse et al, 2020) that among this cells, liver endothelial cells and macrophages from liver and other tissues are the main population going into p16 activation. We are now analyzing the characteristics of p16High tissue resident peritoneal macrophages to understant the roll they have during adulhood and aging. Overall design: F4/80+ macrophages from peritoneal cavity of a 1 year old mice were isolated by magnetic separation (MACS-magnetic beads). Then, the population was separated in EGFP (p16High cells) or tdTomato (p16Low cells) by fluorescence-activated cell sorting, FACS).RNA was isolated and sequencing. Comparative analysis was done comparing both population (p16high vs p16Low). *************************************************************** Raw data files could not be provided for these records. ***************************************************************