Histone acetyltransferase p300/CBP regulates reproductive diapause via the juvenile hormone pathway in the cabbage beetle, Colaphellus bowringi

Juvenile hormone (JH) serves as a pivotal endocrine regulator of reproductive diapause in insects, though the molecular mechanisms governing JH signaling remain poorly characterized. This investigation assesses the roles of the histone acetyltransferase p300/CBP in mediating reproductive diapause through JH signaling in the cabbage beetle Colaphellus bowringi. RNA interference-mediated suppression of p300/CBP in reproductive female adults induced diapause-like phenotypes, including arrested ovarian maturation, suppressed vitellogenesis, enhanced lipid storage, and upregulated expression of diapause-associated genes. Along with downregulation of key JH biosynthetic enzymes and signaling components. While exogenous JH application decreased lipid accumulation, it failed to rescue ovarian developmental arrest. Parallel experiments in diapausing females with p300/CBP knockdown yielded similar results - JH analog treatment reduced lipid reserves without stimulating ovarian development. These ..., , # Histone acetyltransferase p300/CBP regulates reproductive diapause via the juvenile hormone pathway in the cabbage beetle, Colaphellus bowringi

## General information

This dataset does not include large-scale datasets such as transcriptomes; instead, it is organized according to the figures and tables in the article. It primarily comprises RT-qPCR data and various biochemical assay results. The calculated data from RT-qPCR, TG, TL, WB, ovarian size measurements, lipid droplet size measurements, and HPLC/MSMS are provided in CSV format, while other incompatible data are presented in PPT files. Additionally, anatomical images of the cabbage beetle ovaries are included.

**Corresponding author information**

Name: Xiao-Ping Wang
ORCID: [https://orcid.org/0000-0002-1718-1026](https://orcid.org/0000-0002-1718-1026)
Affiliation: Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology, Huazhong Agricultural University, W...,