Table_3_A non-targeted metabolomics analysis identifies wound-induced oxylipins in Physcomitrium patens.xlsx

Plant oxylipins are a class of lipid-derived signaling molecules being involved in the regulation of various biotic and abiotic stress responses. A major class of oxylipins are the circular derivatives to which 12-oxo-phytodienoic acid (OPDA) and its metabolite jasmonic acid (JA) belong. While OPDA and its shorter chain homologue dinor-OPDA (dnOPDA) seem to be ubiquitously found in land plants ranging from bryophytes to angiosperms, the occurrence of JA and its derivatives is still under discussion. The bryophyte Physcomitrium patens has received increased scientific interest as a non-vascular plant model organism over the last decade. Therefore, we followed the metabolism upon wounding by metabolite fingerprinting with the aim to identify jasmonates as well as novel oxylipins in P. patens. A non-targeted metabolomics approach was used to reconstruct the metabolic pathways for the synthesis of oxylipins, derived from roughanic, linoleic, α-linolenic, and arachidonic acid in wild type, the oxylipin-deficient mutants of Ppaos1 and Ppaos2, the mutants of Ppdes being deficient in all fatty acids harboring a Δ6-double bond and the C20-fatty acid-deficient mutants of Ppelo. Beside of OPDA, iso-OPDA, dnOPDA, and iso-dnOPDA, three additional C18-compounds and a metabolite being isobaric to JA were identified to accumulate after wounding. These findings can now serve as foundation for future research in determining, which compound(s) will serve as native ligand(s) for the oxylipin-receptor COI1 in P. patens.

植物氧脂类是一种由脂质衍生的信号分子，参与调节多种生物和非生物胁迫响应的调节。氧脂类的主要一类为环状衍生物，其中包含12-氧代植二烯酸（OPDA）及其代谢物茉莉酸（JA）。尽管OPDA及其较短的链状同系物二去氢-OPDA（dnOPDA）似乎在从苔藓植物到被子植物的陆生植物中普遍存在，但JA及其衍生物的存在尚存争议。在过去十年中，苔藓植物Physcomitrium patens因其作为非维管植物模型生物体的特性而受到科学界的广泛关注。因此，我们通过代谢指纹图谱追踪了损伤后的代谢途径，旨在识别P. patens中的茉莉酸及其新型氧脂类。采用非靶向代谢组学方法重构了由粗糙酸、亚油酸、α-亚麻酸和花生四烯酸在野生型、Ppaos1和Ppaos2的氧脂类缺陷突变体、Ppdes突变体（缺乏所有含有Δ6-双键的脂肪酸）以及Ppelo的C20-脂肪酸缺陷突变体合成的氧脂类代谢途径。除了OPDA、异构-OPDA、dnOPDA和异构-dnOPDA外，还鉴定出三种额外的C18化合物和与JA同位素的代谢物，在损伤后积累。这些发现现在可以成为未来研究的基础，以确定哪种（些）化合物将成为P. patens中氧脂类受体COI1的天然配体。