Information for the 51 Spanish and 1 Portuguese isolates included in the study.

Brachyspira hyodysenteriae isolate names, date of isolation and origin are indicated when these were available. Sequence type (ST) and amino acid type (AAT) in MLST, and their allelic profile (nucleotide/amino acid) are also shown.aIsolates from the same farm are marked with letters A, B, J, H and I in brackets.bFor most of the isolates the Spanish administrative region is specified. Abbreviations used for two regions are: C. Valenciana, Comunidad Valenciana; C-La Mancha, Castilla-La Mancha.cMost of the isolates were recovered from white pigs, but some were obtained from Iberian pigs.dThe month-year of isolation is marked.Isolate H76 was recovered from a pig experimentally infected with US reference strain B204R.Numbers in bold in the allelic profiles correspond to sequences previously described [1], [18].