Transcriptional analysis of primary astrocytes from human fœtal brain inflamed or not with IL-1 beta and subsequently exposed to IL-27

To examine the broad impact of IL-27 on human astrocytes, we performed a microarray analysis assessing >20,000 well annotated genes on primary astrocytes culture from four fetal brain donors. Astrocytes were inflamed or not using human recombinant IL-1 beta (20 ng/mL) befor being exposed or not to human recombinant IL-27 (100 ng/mL). Our goal was to investigate the impact of interleukin-27 on the gene expression profil of human astrocytes Human astrocytes from 4 donors were isolated and then inflamed or not with IL-1 beta for 48h before adding or not of IL-27 (100 ng/mL) in the media. RNA was purified and transcriptome-wide analysis of gene expression was performed by Genome Québec using the Clariom S HT array.