A multiOMICs approach shed light on the higher yield phenotype and abiotic stress tolerance in tobacco lines expressing the carrot lycopene β-cyclase1

Recently we published a set of tobacco lines, expressing the Dacucus carota (carrot) DcLCYB1 gene, with accelerated development, increased carotenoid content, and plant yield. Due to this, expression of DcLCYB1 might be of general interest in crop species as a strategy to accelerate plant development and increase biomass production under adverse field conditions. However, to follow this path, a better understanding of the molecular basis of this phenotype is essential. Here, we combine OMICs (RNAseq, proteomics, metabolomics, and lipidomics) and stress experiments to advance our understanding on the broader effect of the LCYB expression on the tobacco genome and metabolism. Overall 12 samples were analyzed including 3 transgenic lines and 1 wild type (control) and each sample consist of 3 biological replicates.