Sequencing results of vectors contains containing phototropin CDSs for expression in insect cells and bacteria

Results of sequencing of  vectors with phototropin CDS:

You can view all sequencing results as assembled contigous sequence of mutants of phototropin1 or phototropin2 at Gene Snap Viewer (www.snapgene.com/snapgene-viewer).

Phototropins sequences represented by: phot1 CDS, phot2 CDS

phot1 CDS – 2991 nucleotides

phot2 CDS – 2748 nucleotides

phot2LOV1LOV2 - N-terminal PHOT2 fragment, containing LOV1 and LOV2 – 1161 nucleotides

Mutants:

Phot1

1_pFASTphot1D806N – mutation of 1 nucleotide at 2416 position,

2_pFASTphot1C234A – mutation of 2 nucleotides at 700 and 701 positions,

3_pFASTphot1C512A – mutation of 2 nucleotides at 1534 and 1535 positions,

4_pFASTphot1C234AC512A – mutation of 2 nucleotides at 700 and 701 as well as at 1534 and 1535 positions,

Phot2

5_pFASTphot2D720A – mutation of 1 nucleotide at 2158 position,

6_pFASTphot2C170A – mutation of 2 nucleotides at 508 and 509 positions,

7_pFASTphot2C426A – mutation of 2 nucleotides at 1276 and 1277 positions,

8_pFASTphot2C170AC426A – mutations of 2 nucleotides at 508 and 509 positions as well as at 1276 and 1277,

phot2LOV1LOV2 fragment mutants

9_pDONR221phot2LOV1LOV2V392T – mutation of 2 nucleotides at 799 and 800 positions,

10_pDONR221phot2LOV1LOV2V392L – mutation of 1 nucleotide at 799 position,

Phot2 without stop codon (2745 nucleotides)

11_pDONR221phot2V392T – mutation of 2 nucleotides at 1174 and 1175 positions,

12_pDONR221phot2V392L – working on

Phot2 with stop codon (2748 nucleotides)

13_pDONR221phot2V392Tstop – mutation of 2 nucleotides at 1174 and 1175 positions,

14_pDONR221phot2V392Lstop – mutation of 1 nucleotide at 1174 position.

Phot2 574aa stop (1722 nucleotides) 

15_pDONR221phot2 574aaastop – wild type,

16_pDONR221phot2 574aaastop V392T – mutation of 2 nucleotides at 1174 and 1175 positions,

17_pDONR221phot2 574aaastop V392L – mutation of 1 nucleotide at 1174 position.