Designer high-density lipoprotein particles enhance endothelial barrier function and suppress inflammation

High-density lipoprotein (HDL) nanoparticles promote endothelial cell (EC) function and suppress inflammation, but their utility in treating EC dysfunction has not been fully explored.  Here, we describe a fusion protein named ApoA1-ApoM (A1M) consisting of apolipoprotein A1 (ApoA1), the principal structural protein of HDL that forms lipid nanoparticles, and ApoM, a chaperone for the bioactive lipid sphingosine 1-phosphate (S1P). A1M forms HDL-like particles, binds S1P, and is signaling competent. Molecular dynamic simulations showed that the S1P-bound ApoM moiety in A1M efficiently activated the EC surface receptors. Treatment of human umbilical vein endothelial cells (HUVECs) with A1M-S1P stimulated barrier function either alone or cooperatively with other barrier-enhancing molecules, including the stable prostacyclin analog iloprost, and suppressed cytokine-induced inflammation. A1M-S1P injection into mice during sterile inflammation suppressed neutrophil influx and inflammatory medi..., , , # Designer high-density lipoprotein particles enhance endothelial barrier function and suppress inflammation

[https://doi.org/10.5061/dryad.z8w9ghxm4](https://doi.org/10.5061/dryad.z8w9ghxm4)

All the datasets, western blot images, Excel files, Prism files, and statistical analyses related to the figures presented in this paper were stored. Empty cells in the Prism files indicate null values, and they can not be replaced by \"null\" or \"n/a\" because it interfere with the Prism analysis. If anyone has a question after reviewing the raw data and analysis, please contact the first and corresponding authors. 

## FILE LIST AND DATA-SPECIFIC INFORMATION

1. **Fig 1A Purified A1M 4ug 10ug ApoMFc ApoMFc-TM (Original gel).tif**

Purified A1M (4 μg and 10 μg), ApoM-Fc (4 μg), and ApoM-FC-TM (4 μg) from CHO cell-conditioned media were separated by reducing 10% SDS-PAGE and stained with Coomassie Brilliant Blue. 
2. **Fig 1B_S1P content in A1M-S1P.pzf**

S1P content of A1M-S1P was analyzed b...