Anna Baccei, Susan Lancelle (2011) CIL:12599, Canis lupus familiaris, epithelial cell. CIL. Dataset

Network of stress fibers in an MDCK cell. This transmission electron micrograph of a flat-embedded MDCK cell was taken 60-70nm from the interface between the cell and the coverslip on which it was grown and fixed. The cells were cultured on Thermanox® coverslips, then chemically fixed with glutaraldehyde and osmium tetroxide before being stained en bloc with uranyl acetate. The cells were infiltrated with Spurr's® resin, placed under a vacuum for fifteen minutes, and then baked at 70° C. The coverslips were peeled away from the hardened resin wafers, which were then cut and thin-sectioned with a diamond knife. The 65 nm sections were placed on grids and post-stained with uranyl acetate and lead citrate. They were imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV. Because the section was taken just inside the plasma membrane, many vesicles can be seen in this image, particularly in the lower left corner. A neighboring cell in the upper right is shown to underlap the main cell in this image. Scale bar = 2µm.

MDCK细胞中的应力纤维网络。本图为一幅扁平嵌入的MDCK细胞透射电子显微镜图像，拍摄距离细胞与生长并固定其上的盖玻片界面60-70纳米。细胞培养于Thermanox®盖玻片上，随后用戊二醛和四氧化锇进行化学固定，并使用铀酰乙酸进行整体染色。细胞经Spurr's®树脂浸透，置于真空状态下十五分钟，然后在70°C下烘烤。将盖玻片从硬化的树脂晶圆上剥离，随后用金刚石刀切割并制备成薄切片。65纳米的切片放置于网格上，并使用铀酰乙酸和柠檬酸铅进行后染色。使用Philips CM 100透射电子显微镜，在80kV的加速电压下进行成像。由于切片是在质膜内侧取材，因此在该图像中可以观察到许多囊泡，尤其是在左下角。右上角的一个邻近细胞显示与主细胞部分重叠。比例尺=2微米。