Single-cell RNA-sequencing combined with TCR-sequencing of GP66+ splenic CD4+ T cells on day 10 post LCMV Cl13 infection

Here, we applied scRNA and TCR-seq approaches to determine the heterogeneity and clonal landscape of GP66+CD4+ T cells during LCMV clone 13 infection Overall design: GP66-specific CD4+ T cells were FACS-sorted from two indiviual LCMV Cl13-infected C57BL/6 mice on day 10 p.i. Samples were loaded on the Chromium Controller (10x Genomics). Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 5' v2 Reagent Kit (10x Genomics) according to manufacturer's protocol.