Supplementary file 1_Natural vs. genetically engineered microbiomes: understanding public attitudes for indoor applications and pathways for future engagement.docx

This study examines public preferences for natural microbiomes and support for genetically engineered (GE) microbiomes in the built environment, focusing on the demographic, sociographic, and attitudinal factors that influence these preferences. Using data from a nationally representative survey of 1,000 U.S. adults, we employed hierarchical regression analyses to assess the relative contribution of these variables. While demographic and sociographic factors explained limited variance, topic-specific attitudes, including positive perceptions of microbiome engineering’s potential to improve quality of life, were the most significant predictors of support. Conversely, age, distrust in science, and perceived knowledge negatively influenced support for GE microbiomes, reflecting skepticism among some audiences. The findings highlight the potential of the Responsible Research and Innovation (RRI) framework to align the development of microbiome engineering with societal values and to address diverse public perspectives. This research provides actionable insights for policymakers, researchers, and communicators seeking to navigate the complexities of public engagement with emerging biotechnologies.