Protocols forprotein-DNA binding analysis of azinc finger transcription factor bound to its cognate promote
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Here,wedescribeprotocolstointerrogatethebindingofthezincfingersofthe transcriptionfactorZBTB7Atothefetalg-globin(HBG)promoter.Wedetailthe stepsforperformingelectrophoreticmobilityshiftassays(EMSAs),X-raycrystal-lography,andisothermaltitrationcalorimetry(ITC)toexplorethisinteraction.Thesetechniquescouldreadilybeappliedtothestructuralstudiesofother zincfingertranscriptionfactorsandcognateDNAsequences.
Forcompletedetailsontheuseandexecutionofthisprotocol,pleasereferto Yangetal.(2021).
提供机构:
中国科学技术大学
创建时间:
2023-11-23



