T cell signaling proteins 3D confocal microscope movies

This
dataset contains 3D confocal microscope movies of T cell clones expressing
different eGFP-tagged proteins as they undergo synapse formation with antigen
presenting cells (APCs). Each movie shows a field of T cells and APCs every 20
s for 6 minutes after the cells are mixed. Each frame shows some cells in various stages
of antigen presentation. The movies are
for two parental T cell clones, four stimulation conditions and sixteen
proteins. The acquisition and analysis
of the movies is described in

D. J. Clark, L.E. McMillan, S.L. Tan, G. Bellomo, C. Massou, H. Thompson, L. Mykhaylechko, D. Alibhai, X. Ruan, K.L. Singleton, M. Du, A.J. Hedges, P.L. Schwartzberg, P. Verkade, R.F. Murphy, and C. Wülfing (2019) Transient protein accumulation at the center of the T cell antigen presenting cell interface drives efficient IL-2 secretion. eLife 8:e45789. https://doi.org/10.7554/eLife.45789