Cytocompatibility of 3T3 fibroblast and HUVECs

Representative live/dead staining shows high viability of cells seeded on the dECMMA scaffold, with live cells stained green and minimal dead cells (red), indicating high cytocompatibility.Confocal microscopy imaging of actin filaments (green) and nuclei (DAPI, blue) demonstrates robust cellular spreading, adhesion, and cytoskeletal organization.Immunofluorescent staining of CD31 illustrates HUVEC organization and formation of vasa vasorum-like structures (indicated with yellow arrows); nuclei stained with DAPI (blue).Quantitative metabolic activity assessed by Alamar Blue assay comparing human umbilical vein endothelial cells (HUVECs) and fibroblasts (3T3) proliferation within dECM scaffolds over time, confirming favorable cellular viability and metabolic activity