Localization of EhARPC1 in E. histolytica trophozoites.

(A)E. histolytica cells (without RBC and other cells) were stained for EhARPC1, EhTMKB1-9 (as membrane marker) and actin using mouse anti-EhARPC1 antibody, rabbit anti-EhTMKB1-9 antibody and TRITC conjugated phalloidin respectively, followed by appropriate second antibody labeled with Alexa488 or Alexa-555. (B) Quantitative analysis of colocalisation of EhARPC1 with either EhTMKB1-9 or actin based on ten stained images as in (a) was carried out using Pearson’s correlation coefficient (r). (C) Immunolocalization of GFP-tagged EhARPC1 with respect to actin. Cells expressing GFP-EhARPC1 were harvested and fixed followed by staining with anti-GFP antibody and appropriate secondary antibody conjugated with Alexa 488. Actin was stained using TRITC phalloidin. (Scale bar, 10μm; DIC, differential interference contrast).