ZBTB24 is a conserved multifaceted transcription factor at genes and centromeres that governs the DNA methylation state and expression of satellite repeats [RRBS]

ZBTB24 has emerged as a key player in DNA methylation, immunity and development. To explore these multiple facets of ZBTB24 functions, we performed and integrated multi-omics analyses in mouse and human cellular systems relevant for the physiopathological features of the ICF2 syndrome, a rare genetic disease caused by ZBTB24 mutations. Overall design: DNA methylation profiles were performed and compared by Reduced representation bisulfite sequencing (RRBS) in mESCs cells wild-type or mutant for ZBTB24. The study was conducted during the mESCs cell-state transition : in mESCs maintained in 2i conditions (2i) and after 3 days of differentiation (d3).