Additional file 2: of Transcriptional profiling of swine mammary gland during the transition from colostrogenesis to lactogenesis using RNA sequencing

Provides an aggregate summary of RNA sequencing and alignment for all the samples. mRNA libraries were sequenced on a HiSeq2500 (Illumina Inc.). Quality control metrics were performed on raw sequencing reads using the FASTQC v0.11.15 application. An index of the reference genome was built and single-end clean reads for each individual were aligned to the reference genome by STAR (v2.5.1b). Reads were mapped and annotated to the Sus scrofa genome (v10.2.86), downloaded from EnsemblGenome website (Nov. 2016). Reads aligned were quantified with Subread package (v1.5.0) based on the Refseq gene annotation. (XLSX 10Â kb)