Species-specific progenitor domain extends neurogenesis and increases motor neuron production

Human and mouse cells undergoing in vitro motor neurogenesis were sampled at high temporal resolution to identify species' differences in the progenitor pools that give rise to disproportionate timescale changes of motor neurogenesis between human and mouse. Human iPS cells and mouse ES cells were subjected to in vitro motor neuron differentiation, which recapitulates species-specific timescales of motor neurogenesis. Cells were sampled every 24 (mouse) and 48 (human) hours during neurogenesis and profiled using single-cell RNA-seq (3').