Assembly types in crystal structures with tiling BMC-H.

In total, 54 structures of BMC-H were extracted from the RCSB databank and inspected. Only hits presenting planar arrangements of hexamers or 1D-stripped organizations were retained. Wild-type BMC-H entries studied herein by MD simulations are indicated in black bold letter. Non studied cases appear in grey and include data from mutants. A few other cases presenting planar arrangements were excluded from the table because contacts were either too weak or did not follow canonical arrangements: PduASent (4RBT), PduUSent (3CGI), CmcBEcol (7MPW), CutNSint (7MMX), CcmK2Telo (3SSR) or BMC-HHoch (6NLU). The 5th and 6th column report distances measured between alpha carbons of either Lys26 or Arg79 (PduASent) interfacial residues from interacting hexamers (corresponding residues in other BMC-H or mutants). The second portion of the table provides information on BMC-H organizations present in structures from reconstituted BMC. Only the first one (5V74) was obtained by crystallographic methods, all other by cryo-EM. (XLSX)