Acer japonicum transcriptome analysis during autumn leaf senescence

This project aimed to analyze the transcriptomic changes in sun and shade leaves of Acer japonicum (fullmoon maple) during autumn leaf senescence. This project included isoform sequencing (Iso-seq) analysis to obtain full-length cDNA sequences and RNA-seq analysis using DNBSEQ reads. Three leaves from the north-faced outer canopy (sun leaves), and another three leaves from the north-faced inner canopy (shade leaves) of an adult A. japonicum tree (height: approximately 10 m, age: 50 years old) grown in the arboretum of Hokkaido Research Center, Forestry and Forest Products Research Institute (43.0°N, 141.4°E; 180 m a.s.l.) were used for the total RNA extraction. The leaves were harvested on July 7, July 17, and September 7, 2021, for Iso-seq analysis and on September 7, September 28, October 5, and October 13, 2021, for transcriptome analysis.