Genome of a male Chrysoperla carnea

High-molecular-weight DNA was extracted from whole body tissue of one male C. carnea, referred to as BM3, using the standard Qiagen MagAttract HMW kit protocol. HiFi sequencing libraries for BM3 were prepared using the Pacific Biosciences protocols for low DNA input using SMRTbell Express Template prep kit 2.0 with the exception of nuclease treatment during the library step. DNA was fragmented using Megarupter 3. The library was sequenced on one 8M SMRT cell on a Sequel II instrument using Sequel II Binding kit 2.0 and sequencing chemistry v2.0. Loading was performed by diffusion, with pre-extension for 2 hours and a movie time of 30 hours. CCS sequences were generated using the CCS pipeline (SMRT Link v9.0.0.92188) with minimum number passes of 1 and minimum predicted accuracy of 0.99. Cutadapt v3.4 was used to remove any remaining adapter sequences before genome assembly was done using Hifiasm v0.15-r327.