Harr Gupta et al. raw data

Bleomycin was intratracheally delivered to mouse lungs to induce fibrosis. At different time points post-bleomycin, precision-cut lung slices (PCLS) from fibrotic mouse lungs were incubated with radiolabeled peptide ([64Cu]Cu-PEG-FUD) and radioactivity was quantified via gamma counting and autoradiography. Alternatively, at different time points post-bleomycin, fluorescently labeled peptide (Cy5-PEG-FUD) was delivered to mice subcutaneously, followed by relevant organ ex vivo IVIS fluorescence imaging and signal quantification. Additionally, lung sections were stained with Mason's trichrome staining and subsequently subjected to Ashcroft scoring to determine the level of fibrotic burden.