Bovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment.

Data from ProteomeXchange, PXD ID: PXD001096. File: 130508_01_Orbi1_SK_SER_G150_T158_BOSS_2xenrich_repl1_try.mzml. Published as part of Proteomics. 2015 Jan 20 . From the Abstract: {{i}} A significant proportion of mammalian fertilization is mediated through the proteomic composition of the sperm surface. These protein constituents can present as biomarkers to control and regulate breeding of agricultural animals. Previous studies have addressed the bovine sperm cell apical plasma membrane proteome with nitrogen cavitation enrichment. Alternative workflows would enable to expand the compositional data more globally around the entire sperm"s surface. We used a cell-surface biotin-labelling in combination with differential centrifugation to enrich sperm surface proteins. Using nano-LC MS/MS, 338 proteins were confidently identified in the plasma membrane enriched proteome ... {{/i}}