Results of PCR and subsequent sequencing of PCR products positive for deformed wing virus (DWV).

Two tubes were obtained from each colony for initial identification; these were each analysed twice for a total of four PCRs per colony. If both tubes were positive, relative gene expression is provided for both.1A DWV band was obtained in the earlier gel, but not in a later gel; possibly sample degradation. Sequencing not attempted.2One of two samples from the colony was positive for DWV in both analysis; DWV presence confirmed in second analysis.3RT-PCR was negative for DWV in both first and second gel but RT-qPCR showed very low levels of DWV. Sample was not sequenced; thus DWV presence was not confirmed.4No DWV bands were obtained in the first analysis, but one band was obtained in the second analysis, and presence was confirmed by sequencing.5In one of two sample, no DWV band was obtained in the first analysis, but a band was obtained in the second analysis, and presence was confirmed by sequencing. Second sample from this colony was positive both times.6DWV bands were obtained in the first gel for both colonies NF103 and NF112 but one of the two bands was faint for each colony; in the second analysis no band was observed from the sample with a faint band. The second analysis confirmed DWV was present (by sequencing) in both colonies.