Inhibition of signaling pathways that are involved in MLC phosphorylation or inhibition of actin polymerization led to dose-dependent restriction in influenza virus proliferation.

MDCK cells were treated with the solvent used for dissolving the chemicals, DMSO or PBS (control) or serial dilutions of the indicated chemicals for the indicated time. The cells were infected with influenza A virus (MOI, 0.01) and 24 h after the infection, RT-PCR was used to measure the virus yield in the medium. The proliferation of influenza virus was inhibited in a dose-dependent manner for all chemicals (graphs not shown). N = 4 for each experiment.