DROSHA complementation experiments using small RNA sequencing. DROSHA complementation experiments using small RNA sequencing

DROSHA complementation experiments were performed to check whether dsRBD of DROSHA recognizes the lower stem of pri-miRNAs and affects miRNA biogenesis. Overall design: Mature miRNAs were sequenced from HCT116 DROSHA-null cells which had been transfected with wild-type or mutant DROSHA plasmids.