African swine fever infection epigenetically regulate host membrane protein-encoding genes

African swine fever virus (ASFV) is a highly contagious pathogen that primarily affects domestic and wild pigs with specific tropism to porcine alveolar macrophages (PAMs). However, the host receptors involved in ASFV infection remain unknow. Here, we present a multi-omic epigenetic atlas of ASFV-exposed PAMs and profile 3D chromatin architecture and single-nucleus chromatin accessibility landscapes (sn-ATAC) AMs with ASFV and assessed gene expression (RNA-seq),Genomic DNA samples from ASFV infected PAMs and uninfected controls were then analyzed for multiple chromatin modification states by CUT&Tag (H3K4me3, H3K27ac, H3K27me3 and Pol2),sn-ATAC to map the transposase-accessible chromatin at the single-cell level,Through a high-resolution chromatin conformation capture technique(BL-HiChIP)