Data for: The 3-dimensional genome drives the evolution of asymmetric gene duplicates via enhancer capture-divergence

Previous models of duplication-based new gene evolution have overlooked the role of genome architecture. The enhancer-capture divergence model provides a one-step mechanism for altering the expression of a pre-existing protein. By duplicating into a region of the genome that is under the influence of a pre-existing enhancer, distal duplication of an existing gene provides for immediate augmentation of spatiotemporal expression of the existing gene. In the related manuscript, we examine the local chromosomal conformations for a newly evolved gene HP6/Umbrea, which appears to have undergone the enhacer-capture divergence process. While all data from the associated manuscript are publicly available through permanent NCBI repositories, additional related 4C-Seq data was generated but did not appear in the final manuscript. The following data set consists of 1) raw reads from 4C-Seq experiments performed on D. melanogaster, D. yakuba, D. erecta, and D. pseudoobscura for HP6/Umbrea and a neig..., Imaginal disc tissue from 4 species was extracted from L3 wandering larvae. A standard 4C-Seq protocol was performed with a primary digest of DpnII and a secondary digest of Csp6i. Further details may be found in the associated manuscript., , # Data for: The 3-dimensional genome drives the evolution of asymmetric gene duplicates via enhancer capture-divergence

## Description of the data and file structure

Raw reads for S2 cells (UL-4C_S2_L002_R1_001.fastq) and imaginal disc (UL4-PhIX_S1_R1_001.fastq) are provided. Barcodes for de-multiplexing are provided (4c_s2_barcodes.txt for S2, barcode.txt for imaginal disc). 4C-Seq for S2 cells was performed for CG7041 and HP6/Umbrea. 4C-Sq was performed for imaginal disc tissue for HP6/Umbrea and neighboring locus (see related manuscript) in *D. melanogaster*, *D. yakuba*, *D. erecta*, and *D. pseudoobscura* (future insertion locus). Scripts for Lee, Arsala, and Xia et al. are also provided.

Data was generated from:

* S2 cells
* *D. melanogaster* imaginal disc tissue
* *D. yakuba* imaginal disc tissue
* *D. erecta* imaginal disc tissue
* *D. pseudoobscura* imaginal disc tissue

## Code/Software

Scripts for all analyses.

## Access information

Other publicly accessible locations ...