LncRNA and mRNA expression profiles in High-grade serous ovarian cancer, Low-grade serous ovarian cancer and Normal fallopian tube

Ovarian cancer is the most lethal gynecologic cancer. High-grade serous ovarian carcinoma (HGSOC) is the most common histologic subtype, accounting for three quarters of ovarian cancer. To clarify the changes of gene expression in serous ovarian cancer, we performed lncRNA and mRNA microarrays to identify differentially expressed lncRNAs and mRNAs in High-grade and Low-grade serous ovarian carcinoma compared with Normal fallopian tube. The High-grade and Low-grade serous ovarian carcinoma specimens were from primary ovarian cancer patients with no surgery or chemotherapy previously and fallopian tube tissues were from patients who received a total hysterectomy and bilateral salpingo-oophorectomy for uterine diseases or benign neoplastic adnexal pathologic changes. Fresh tissue samples were collected within 2 hours of surgery. Total RNA was extracted with Trizol and subjected to microarray assay.