Glycoform quantification of PrPres from the Canadian BSE cases using core antibody 6H4.

The proportion of PrPres detected as diglycosylated is approximately 60% for the C- and H-type. In comparison, the L-type had a much lower proportion of the diglycosylated PrPres at approximately 40%. The L-type case also had a ratio of di- to monoglycosylated (D/M) PrPres well below 2 (1.1), whereas the ratios of intensity of D/M from all other cases were above or equal to 2 when using core specific antibodies for detection. Profiles were determined by analyzing western blot reactivity using the BioRad Quantity One software (BioRad, Canada).D = Diglycosylated PrPres; M = Monoglycosylated PrPres; Un = Unglycosylated PrPres; D/M = Diglycosylated PrPres/Monoglycosylated PrPres.