Supporting data for "A New and Improved Genome Sequence of Cannabis sativa"

<i>Cannabis</i> is a diploid species (2n = 20), the estimated haploid genome sizes of the female and male plants using flow cytometry are 818 and 843 Mb respectively. Although the genome of <i>Cannabis</i> has been sequenced (from hemp, wild and high-THC strains), all assemblies have significant gaps. In addition, there are inconsistencies in the chromosome numbering which limits their use. A new comprehensive draft genome sequence assembly (~900 Mb) has been generated from the medicinal <i>Cannabis</i> strain Cannbio-2, that produces a balanced ratio of cannabidiol and delta-9-tetrahydrocannabinol using long-read sequencing. The assembly was subsequently analysed for completeness by ordering the contigs into chromosome-scale pseudomolecules using a reference genome assembly approach, annotated and compared to other existing reference genome assemblies. The Cannbio-2 genome sequence assembly was found to be the most complete genome sequence available based on nucleotides assembled and BUSCO evaluation in <i>Cannabis sativa</i> with a comprehensive genome annotation. The new draft genome sequence is an advancement in <i>Cannabis</i> genomics permitting pan-genome analysis, genomic selection as well as genome editing.