The binding of Rev, RSG-1.2 and RSG-1.2 mutant peptides with RRE-IIB at 25°Ca.

aThe values of N1, Ka1, ΔH1, TΔS1, N2, , Ka1, ΔH2, and TΔS2 listed here were derived from the fits of the ITC profiles shown in Fig. 6b with a model for two set of binding sites [36]. The indicated uncertainties reflect the standard deviations of the experimental data from the fitted curves (depicted as solid line in Fig. 6b).bValues have been calculated from fluorescence binding studies and standard relationship ΔGb = -RT lnKab have been used to calculate the free energy of the binding.cValues of ΔS were calculated using the standard relationship ΔG = ΔH - TΔS, with the indicated uncertainties reflecting the maximum possible errors in ΔH and ΔG as propagated through this equation. ND values not determined. ITC profiles of mutant peptides have been shown in Figures S3 and S4.