Global transcriptome of bone marrow niche cells in homoestatis and human xenografts at late time points

We analysed the transcriptional signature in different niche cells extracted from the bone marrow of mice engrafted with human AML patient-derived samples or AML cell lines and compared it to the one of mice engrafted with human normal hematopoietic cells derived from cord blood or untransplanted mice Overall design: Immunodeficient mice were transplanted with human AML cell lines, human AML cells derived from patients (blood or bone marrow), or with normal human hematopoietic cells (cd34+) derived from cord blood. Mice were sacrificed once assessed the bone marrow engraftment, and the bones were processed to isolate niche cells using the depicted marker. RNA was extracted, sequencing libraries were prepared and sequenced.