Total fatty acid content quantification (relative %).

GC-MS was used to determine the fatty acid content of the different L. infantum strains, in comparison with wild-type parasites. S7 Fig includes an example for total ion chromatogram of derivatised fatty acids from lipid extracts of L. infantum 263 wild-type. Data are the mean of three independent experiments. Statistical significance between the mock-transfected wild-type and the rest of the strains was evaluated by unpaired two-tailed t test (***p ≤ 0.001).