Diplocarpon rosae isolate:DortE4 Transcriptome or Gene expression

To get an overview of the transcriptome of Diplocarpon rosae's early infection stages, MACE (Massive Analysis of cDNA Ends) data were generated for three time points (0, 24, 72 hpi). To generate three biological repetitions independent inoculation experiments for the incompatible interaction of the isolate DortE4 with the susceptible rose variety “Pariser Charme” were performed. To expand the analysis, RNA of the three samples at 72 hpi were also used for a conventional RNAseq approach. The reads belonging to the fungus were extracted by mapping the datasets to the Diplocarpon rosae draft genome sequence with the CLC Genomics Workbench 9.0.1.