scRNAseq from pbmc, ileum, and rectal tissue of healthy and crohns disease individuals

Crohn’s disease (CD) is a major subtype of inflammatory bowel disease (IBD), a spectrum of chronic intestinal disorders caused by dysregulated immune responses to gut microbiota. We performed Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITEseq) on peripheral blood, colon, and ileal immune cells derived from healthy subjects and patients with Crohn’s disease. Healthy individuals were undergoing colonoscopy as part of routine clinical care for colorectal cancer screening/surveillance or non-inflammatory gastrointestinal symptoms that included constipation or rectal bleeding. Cells were isolated, sorted, partitioned, and sequenced.