Multiple Histone modifications ChIP-seq in MCF7 cells corresponding to Ctr9 KD (paper title: Ctr9, the scaffold subunit of PAFc, govens PRC2-mediated H3K27me3 domains in breast cancer cells)

We want to examine the alternation of histone modification spectrum on chormatin in corresponding to Ctr9 loss either in an inducible KD or stable KD fashion in MCF7 breast cancer cells. We applied 5 samples by indiviaually pulling down Histone H3K4me3/H3K27me3/H3K36me3 enriched DNA followed by deep sequencing. Two samples are MCF7-tet-on-shCtr9 cells either treated with Vehicle (control) or Doxycycline(+ Dox: inducible Ctr9 KD) for 7 days. The other three samples are MCF7 cells stablying expressing scarmbolled shRNA or two distinct lentiviral shRNAs target to Ctr9 transcript. (shCtr9#3/shCtr9#5)