Comparison of microarray expression data from 23 day mouse germ cells from control and Mgat1 conditional knockout mice

Loss of Mgat1 in spermatogonia was investigated in germ cells from 23 day males. Gene expression changes induced by deletion of Mgat1 were determined using the Affymetrix GeneChip Mouse Gene 2.0 ST Array. We include the expression data obtained from three replicate aliquots of pooled testis total RNA obtained from six control or six conditional knockout Mgat1 testis germ cells from 23 dpp males, Three replicates of the respective control and Mgat1 cKO pools were used for microarray analysis using Affymetrix GeneChip Whole Transcript Plus Reagent Kit. We identified differential expressed genes with a significance level of ANOVA and FDR p < 0.05 and an absolute fold-change (linear) less than -2, and greater than 2.0. RNA from 3 replicates of control and 3 replicates of mutant germ cell RNA pools were purified using Trizol and Rneasy minielute clean up kit (Qiagen), RNA integrity was measured and samples with RIN >9.0 were submitted for cDNA preparation and microarray analysis.