Comparative mass-spectrometry of pTyr immunoprecipitated zebrafish lysates.
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Zebrafish embryos were injected at the 1-cell stage with synthetic mRNA constructs encoding WT Shp2, NS (D61G) Shp2 or LS (A462T) Shp2 and co-injected with mRNA encoding eGFP. Lysates were subjected to mass spectrometry as described in Materials and Methods. Normalized ratios (Log2 scale) based on total levels of non-phosphorylated peptides are given.*Protein name based on BLAST sequence of peptide. Accession numbers from BLAST hits are used for non-annotated peptides.**pRS score Figure 1C.Comparative mass-spectrometry of pTyr immunoprecipitated zebrafish lysates.
创建时间：
2015-12-02
