Whole kidney gene expression (mRNA) as measured by quantitative RT/PCR (qRT-PCR) for wild type and HYP mice infused with vehicle or SPR4-peptide (see also Figure 3).

Column headings represent; WT = wild type mice, HYP = X-linked hypophosphatemic rickets mice, SPR4 = infused SPR4-peptide and Vehicle = Saline infused. For gene analysis mice were sacrificed on day 28 and mRNA prepared from whole kidneys as detailed in methods (N = 6). For qRT-PCR gene analysis fold differences in expression calculated by the Pfaffl method [163] were statistically analyzed for significance using the One Sample t-test and the Wilcoxon Signed rank-test with theoretical means set to 1. Results for all tests were considered to be significantly different at pWT-VE versus HYP-VE, WT-VE versus WT-SPR4 and HYP-VE versus HYP-SPR4 are shown in columns 1, 2 and 3 as indicated in the headings. ND = Not done, NS = Not Significant. Index: NPT2a = Sodium-dependent phosphate co-transporter (Slc34a1); NPT2c = Sodium-dependent phosphate co-transporter (Slc34a3); 1-α-Hydroxylase = 25-hydroxyvitamin D3 1-alpha-hydroxylase (CYP27B1); 24-Hydroxylase = 1,25-hydroxyvitamin D3 24-hydroxylase (CYP24A1); VEGF = Vascular Endothelial Growth factor; SOST = Sclerostin; GAPDH = Glyceraldehyde 3-phosphate dehydrogenase; Cyclophilin = peptidylprolyl isomerase A (cyclophilin A).