Design of proteins with desired thermal properties is important for scientific and biotechnological applications. Here we developed a theoretical approach to predict the effect of mutations on protein
Averaged cultivation parameters of CHO shaking flask cultivations. Parameters under three different nutrient-pulsing regimes. Data show condition (A) with only medium spiked, fructose spiked medium wi
The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can sh
Site saturation mutagenesis library is a recently developed technique, in which any one out of all amino acid residues in a target region is substituted into other 19 amino acid residues. In this stud
