Figure 5H-I primary data

Figure 5H-I legend. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) to block IFN-β paracrine signaling. Protein was isolated from BC3-IFN-βp-tdTomato reporter cells without sorting (“bulk”), or after sorting the lytic+casp-i+anti-IFN Abs based on tdTomato expression. Western blots were probed for total RelA and phosphorylated RelA (Ser536) and β-actin as a loading control. Levels of phosphorylated RelA (Ser536) were quantified by normalizing to total RelA and plotted relative to bulk lytic+casp-i+ anti-IFN Abs samples.