BXSB_mice_IgG_glycosylation_batch2

Batch 2.

The folder Batch2 contains mzXML files obtained in a liquid chromatography-mass spectrometry (LC-MS) analysis of Fc-linked IgG N-glycosylation profiles of mouse immunoglobulin G in a BXSB mice cohort, batch 2.
First part of batch1 can be found in the following dataset: 10.17632/jygxpd7g7c.1
Second part of batch1 can be found in the following dataset: http://dx.doi.org/10.17632/kjfw7cwnxf.1
Male BXSB mice spontaneously develop an autoimmune disorder similar to systemic lupus erethematousus (SLE) which involves production of autoreactive antibodies.
We have isolated IgG from serum sampled at 10 and 18 weeks of age in a cohort of sex-matched BXS mice, treated either with anti-CD20 specific antibody that transiently depletes B-cells during the pre-phase of the disease or with an isotype antibody (control). IgG was digested with trypsin, the glycopeptides desalted and enriched by reverse-phase solid phase extraction and analyzed on an LC-MS system.

We observed higher abundance of sialylated IgG1 and IgG2b/IgG2c glycoforms in the male mice treated with CD20-specific antibody as compared to isotype-treated male mice, while no major changes were observed in galactosylated IgG glycoforms between isotype and CD20-specific antibody treated male mice.

Information about glycoforms observed in the samples and corresponding m/z values of analytes can be found in the excel sheet: Supplementary Table S1.xlsx
Description of the samples could be found in the following excel sheet: Supplementary Table S2.xlsx

批次 2。
该文件夹Batch2 包含了通过液相色谱-质谱联用（LC-MS）技术分析BXSB小鼠群体中Fc连接的免疫球蛋白G N-糖基化谱所获得的mzXML文件。批次1的第一部分可参见以下数据集：10.17632/jygxpd7g7c.1，第二部分可参见以下数据集：http://dx.doi.org/10.17632/kjfw7cwnxf.1。
雄性BXSB小鼠自发性地发展出一种类似系统性红斑狼疮（SLE）的自身免疫性疾病，该疾病涉及自反应抗体的产生。
我们从性匹配的BXS小鼠的10周和18周龄血清样本中分离出IgG，这些小鼠在疾病前期分别接受了CD20特异性抗体（暂时耗竭B细胞）或同型抗体（对照组）的治疗。IgG经胰蛋白酶消化，糖肽通过反相固相萃取进行脱盐和富集，并在LC-MS系统上进行分析。
我们观察到，与接受同型抗体治疗的雄性小鼠相比，接受CD20特异性抗体治疗的雄性小鼠中唾液酸化的IgG1和IgG2b/IgG2c糖型丰度更高，而在同型抗体和CD20特异性抗体治疗的雄性小鼠之间，乳糖基化的IgG糖型没有观察到显著变化。
有关样品中观察到的糖型和对应分析物的m/z值的信息，请参阅以下Excel表格：补充表S1.xlsx。样品描述可参见以下Excel表格：补充表S2.xlsx。